Effect of different levels of thyme extract on nitrogen retention, gas production, microbial population, protozoa and some blood parameters in fattening lambs and Kids

Document Type : Research Paper

Authors

1 Ph.D Student, Department of Animal Nutrition,Gorgan University of Agricultural Sciences&Natural Resources

2 Gorgan University of Agricultural and Natural Resources

3 Department of Animal Nutrition,Gorgan University of Agricultural Sciences&Natural Resources

4 Gorgan University of Agricultural Sciences&Natural Resources

5 Research and Education Center for Agriculture and Natural Resources

Abstract

Low feed efficiency is one of the problems in the rumen of ruminants, which results in reduced growth and production of livestock and, most importantly, environmental pollution due to the release of methane and nitrogenous compounds . Changes in ruminal fermentation can be made to improve fermentation efficiency and substrate utilization and optimize feed nutritional value . Antibiotics have been successful in reducing energy loss (from methane) and protein (from ammonia nitrogen) in the rumen . However, today, due to general concerns about the remains of these compounds in milk and meat and thus increasing the bacterial resistance to them has caused the use of materials in feed as additive in some countries . Therefore, the tendency to find alternatives with natural origin for antibiotics has increased over recent years .Plant secondary metabolites are among the natural alternatives that have been studied in recent years that have antimicrobial properties.Essential oils, also known as volatile or ethereal oils, occur in edible, medicinal, andherbal plants. As these aromatic compounds are largely volatile, they are commonly extracted by steam distillation or solvent extraction . Essential oils can be extracted from many parts of a plant, including the leaves, flowers, stem, seeds, roots and bark. However, the composition of the EO can vary among different parts of the same plant . For instance, EO obtained from the seeds of coriander (Coriandrum sativum L.) have a different composition from the EO of cilantro, which is obtained from the immature leaves of the same plant . Chemical differences among EO extracted from individual plants, or different varieties of plants, also exist and are attributed to genetically determined properties, age of the plant, and the environment in which the plant grows . One of the plant essential oils that has a great potential for use in ruminant diets is the essential oil of thyme (Thymus Vulgaris). Thyme extract contains 0.6-8.8% (usually 1%) of essential oil, most of which are phenols (20-20%) and monoterpene hydrocarbons (such as P cymen and γ terpinen) and alcohols (such as Linalool and terpinen α). They show that sometimes these compounds make up 80% of the extract compounds. Naturally, thymol is also a major phenolic component in thyme and carvacrol is a sub – element (Leung and Foster., 1996). Although goats and sheep have often been fed and managed in a similar manner (goats being browsers and sheep being grazers), they are different, especially in their digestive capability, because of differences in the morphology of digestive system, including the mouth structure and gut, which may influence their ability to ingest and digest feed materials . Available information on efficiency of feed utilization between goat and sheep has been inconsistent with several studies reported sheep had higher growth performance than goats . In a recent in vitro study, Candyrine et al (2016) reported that goats had better rumen fermentation characteristics, including higher volatile fatty acids (VFAs) production and higher population of cellulolytic bacteria compared to sheep, suggesting that goats could be more superior in digesting feed materials.The results of studies performed on lambs and goats ) that have examined the effects of thyme extract or its major compounds (thymol and carvacrol) are very contradictory. And more research is needed to determine their effects on rumen fermentation and metabolism in lambs and goats. There is also little research on direct comparisons of the use of plant extracts in vivo between lambs and goats. Therefore, the aim of the present study was to compare these two species and the effects of thyme extract on performance and some ruminal parameters in fattening lambs and goat kids.
The aim of this study was to investigate the effect of different levels of thyme (Thymus vulgaris) extract on growth performance, feed intake, daily weight gain and digestibility of nutrients in fattening lambs and kids. For this purpose, 15 goat kids (average initial BW of 17.3 ± 1.2 Kg, 3 month- old) and 15 Dalagh lambs (average initial BW of 21.4 ± 1.5 Kg, 3 month- old) were randomly assigned to 3 dietary treatments: 1) control (without thyme extract), 2) supplemented with 250 ml thyme extract and 3) supplemented with 500 ml thyme extract. Animals were kept in individual pens with self-mangers for 84 days. The results showed that there was no significant difference in nitrogen consumption through the use of extracts between different treatments. However, fecal and urinary excretion nitrogen was lower in treatments receiving 250 ml of thyme extract and retained and digested nitrogen was more than other treatments (P <0.01). Examination of interactions and species effects showed that the effect of thyme extract on excreted nitrogen and nitrogen retention in lambs was different from that of goat kids, so that excreted nitrogen was higher in goats and nitrogen retention was lower. With increasing levels of thyme extract, a significant decrease in gas and methane production was observed in both species (P <0.01). Rumen microbial population (total bacteria, coliforms and lactic acid bacteria) decreased due to the addition of thyme extract in both species, but this decrease was not significant. Also, in lambs and goats receiving thyme extract, with increasing the levels of the extract in the diet, the number of protozoa decreased significantly (P <0.01), so that the group receiving 500 ml of extract had the lowest number of protozoa. Concentrations of triglycerides, cholesterol, low-density lipoprotein and high-density lipoprotein were not affected by the use of the extract. However, blood glucose concentration increased significantly with increasing the extract in the diet and blood urea nitrogen concentration decreased (P <0.01). In general, the results of this study showed that the use of thyme extract in the diets of lambs and goats can significantly reduce gas production, especially methane gas and excretion of nitrogen through urine and feces, and thus increase nitrogen retention and digestion.

Keywords

References

Agarwal N, Shekhar C, Kumar R, Chaudhar LC and Kamra DN, 2009. Effect of peppermint (Mentha piperita) oil on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor. Animal Feed Science and Technology 148: 321.327.
AOAC, 2005. Official Methods of Analysis. Association of Official Analytical Chemists. Washington, DC. USA.  .
Bampidis VA and Christodouloa V, 2005. Effect of dietary dried oreganomleavels supplementation on performance and carcases characteristics of growing lambs. Animal Feed Science and Technology 121:285-295.
Benchaar C, Calsamiglia S, Chaves AV, Fraser GR, Colombatto D, McAllister TA and Beauchemin KA, 2008. A review of plant-derived essential oils in in ruminant nutrition and production. Animal Feed Science and Technology 145: 209 228.
Biricik H, Oral HH, Taluğ AM, Cengiz SS, Koyuncu M and Dikmen S, 2016. The effects of carvacrol and/or thymol on the performance, blood and rumen parameters, and carcass traits of Merino sheep. Turkish Journal Veterinary Animal Science 40: 651–659.
Bunthoeun P, 2007. Studies on manipulation of ruminal fermentation and methanogenesis by natural products, Ph. D dissertation, Iwate State University, USA.
Busquet M, Calsamiglia A, Ferret A and Kamel C, 2006. Plant extracts affect in vitro rumen microbial fermentation. Journal of Dairy Science 89: 761-771.
Busquet M, Calsamiglia S, Ferret A, Cardozo PW and Kamel C, 2005. Effects of cinnamaldehyde and garlic oil on rumen microbial fermentation in a dual flow continuous culture. Journal of Dairy Science 88: 2508-2516.
Candyrine SCL, Jahromi MF, Ebrahimi M, Chen WL, Rezaei S and Goh YM, Abdullah N and Liang JB, 2019. Oil supplementation improved growth and diet digestibility in goats and sheep fed fattening diet. Asian-Australasian Journal of Animal Science 32(4): 533-540.
Candyrine SCL, Liang JB and Goh YM, 2016. In vitro rumen fermentation characteristics of goat and sheep supplemented with polyunsaturated fatty acids. Animal Production Science 57:1607-12.
Cedrola F, Rossi F, Dias RJP, Martinelli M and Agosto M, 2015. Methods for taxonomic study of rumen ciliates (Aleolata: Ciliofora): a brief review. Zoo Science 32:8-15.
Chowdhury MR, Khan MMH, Mahfuz S U and Baset MA, 2018. Effects of dietary supplementation of spices on forage degradability, ruminal fermentation, in vivo digestibility, growth performance and nitrogen balance in Black Bengal goat. Journal of Animal Physiology and Animal Nutrition 102:591–598.
Cieslak A, Szumacher-Strabel M, Stochmal A and Oleszek W, 2013. Plant components with specific activities against rumen methanogens. Animal 7: 253–265.
Dijkstra J, Oenema O, van Groenigen JW, Spek JW, van Vuuren AM and Bannink A, 2013. Diet effects on urine composition of cattle and N2O emissions. Journal Animal 7: 292–302.
El-Essawy AM, Abdou AR, Khattab IM and Abdel-Wahed AM 2019. Effect of addition of Anise, Clove and Thyme essential oils on Barki lambs performance, digestibility, rumen fermentation, carcass characteristics and intramuscular fatty acids. Egyptian Journal and Nutrition and Feeds 22(3): 465-477.
Ghoorchi T and Seyed Almoosavi SMM, 2018. Ruminant Nutrition Principls. Gorgan University of Agricultural Sciences and Natural Resources 310 pp.
Hess HD, Kreuzer M, Diaz TE, Lascano CE, Carulla JE, Soliva CR and Machmuller A, 2003. Saponin rich tropical fruits affect fermentation and methanogenesis in faunted and defaunated rumen fluid. Animal Feed Science and Technology 109: 79-94.
Hook SE, Wright ADG and McBride BW, 2010. Methanogens: methane producers of the rumen and mitigation strategies. Archaea (Vancouver,B.C.), Animal Production Science 1-11
Hristov AN, McAllister TA, Van Herk FH, Cheng KJ, Newbold CJ and Cheeke PR, 1999. Effect of Yucca schidigera on ruminal fermentation and nutrient digestion in heifers. Journal of Animal Science 77: 2554-2563.
Hristov AN, Ropp JK, Zaman S and Melgar A, 2008. Effects of essential oils on in vitro ruminal fermentation and ammonia release. Animal Feed Science and Technology 144: 55-64.
Hussain I and Cheeke PR, 1995. Effect of dietary Yucca schidigera extract on rumen and blood profiles of steers fed concentrate or roughage-based diets. Animal Feed Science and Technology 51: 231-242.
Jamroz D, Orda J, Kamel C, Williczkiewicz A, Wertelecki T and Skorupin’Ska J, 2003. The Influence of Phytogenic Extract on Performance, Nutrients Digestibility, Carcass Characteristic and Gut microbial Status in Broiler Chickens. Journal of Animal Feed Science 12(3): 583-596.
Kazemi M, 2020.Laboratory study of Balangu (Lallemantia royleana) essential oil effects on gas production kinetic, degradability, protozoan population, and some fermentation parameters of a balanced diet. Journal of Animal Science Research (Agricultural Science) 30:29-43.
Khorrami B, Vakili AR, Danesh Mesgaran M and Klevenhusen F, 2015. Thyme and cinnamon essential oils: Potential alternatives for monensin as a rumen modifier in beef production systems. Animal of Feed Science and Technology 200: 8-16.
Lam E, Rawel H and Schweigert FJ, 2011. The effect of tannins on Mediterranean ruminant ingestive behavior: The role of the oral cavity. Molecules 16: 2766–2784.
Leung AY and Foster S, 1996. Encyclopedia of common natural ingredients used in foods, drugs and cosmetics. A Wiley Inter Science Publication- John Wiley and Sons, Inc, 649 pp.
Lila ZA, Mohammed N, Kanda S, Kurihara M and Itabashi H, 2005. Sarsaponin effects on ruminal fermentation and microbes, methane production, digestibility and blood metabolites in steers. Asian Australasian Journal of Animal Sciences 18:1746-1751.
Martinez S, Madrid J, Hernandez F, Megias MD, Sotomayor JA and Jordan MJ, 2006. Effect of thyme essential oils (Thymus hyemalis and Thymus zygis) and monensin on in vitro ruminal degradation and volatile fatty acid production. Journal of Agricultural and Food Chemistry 54: 6598-6602.
McIntosh FM, Williams P, Losa R, Wallace RJ, Beerver DA and Newbold CJ, 2003. Effects of essential oil on ruminal microorganisms and their protein metabolism. Applied and Environmental Microbiology. 69:5011-5014.
Menke KH and Steingass H, 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development 28: 7.55.
Mirzaei Cheshmehgachi S,  Moeini MM, Hozhabri F and Nooryan Soroor ME, 2017. Effect of essential oils of Zataria multiflora, Eucalyptus globolus and their combination on fermentation parameters using Merghoz goat rumen liquor. Iranian Journal of Applied Animal Science 7(1): 53-59.
Mohammed N, Ajisaka N, Lila K, Mikuni ZA, Hara K, Kanda S and Itabashi H, 2004. Effect of Japanese horseradish oil on methane production and ruminal fermentation in vitro and in steers. Journal of Animal Science 82: 1839.1846.
Morgavi DP, Forano E, Martin C and Newbold CJ, 2010. Microbial ecosystem and methanogenesis in ruminants. Animal, 4 (7): 1024-1036.
National Research Council, 2007. Nutrient Requirement of Small Ruminants. (7th ed). National Academy of Science, Washington, DC.
Newbold CJ, McIntosh FM, Williams P, Losa R and Wallace RJ, 2004. Effects of a specific blend of essential oil compounds on rumen fermentation. Animal Feed Science and Technology 114: 105–112.
Perez-Maldonado RA and Norton BW, 1996. The effects of condensed tannins from Desmodium intortum and Calliandra calothyrsus on protein and carbohydrate digestion in sheep and goats. British Journal of Nutrition 76: 515–533.
Pour Arefi A, Rahchamani R, Ghanbari F and Gharehbash AM, 2016. Effect of essential oil of cinnamon on performance, rumen microbial populations and fermentation and some blood parameters of sheep. Journal of Ruminant Research 4(1): 95-112.
Rogosic J, Pfister JA, Provenza FD, 2006. Sheep and goat preference for and nutritional value of Mediterranean maquis shrubs. Small Ruminant Research 64: 169–179.
Sinz S, Kunz C and Liesegang A, 2018. In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation. Czech Journal of Animal Science 63: 293–304.
Smeti S, Joy M, Hajji H, Alabart JL, Munoz F, Mahouachi M and Atti N, 2015. Effects of Rosmarinus officinalis L. essential oils supplementation on digestion, colostrum production of dairy ewes and lamb mortality and growth. Journal of Animal Science 86: 679–688.
Talatapeh A, Farhoomand P, Aligoo Y, Zahedi M, Ahmadi nagadehi AA and Peyvastegan S, 2014. Effect of summer savory essential oil on performance, rumen fermentation and blood parameters of west Azarbaijan native kids. Animal Science Journal (Pajouhesh & Sazandegi) 105: 179-192.
Talebzadeh R, Alipour D, Saharkhiz MJ, Azarfar A and Malecky M, 2012. Effect of essential oils of Zataria multiflora on in vitro rumen fermentation, protozoal population, growth and enzyme activity of anaerobic fungus isolated from Mehraban sheep. Animal of Feed Science and Technology 172: 115-124.
Terril TH, Douglas GB, Foote AG, Purchas RW, Wilson GF and Barry TN, 1992. Effect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum coronarium) and perennial pasture. Journal Agricultural Science 119:265–273.
Turner KE, Wildeus S and Collins JR, 2005. Intake, performance, and blood parameters in young goats offered high forage diets of lespedeza or alfalfa hay. Small Ruminant Research 59: 15.23.
Van Soest PJ, Robinson JB and Lewis, BA, 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583-3597.
Wallace RJ, McEwan NR, McIntosh FM, Teferedegne B and Newbold CJ, 2002. Natural products as manipulators of rumen fermentation. Asian–Aust. Journal of Animal Science 15:1458–1468.
Wanapat M, Pichad K, Pawadee P and Sadudee W, 2008. Effect of supplementation of garlic powder on rumen ecology and digestibility of nutrients in ruminants. Journal of the Science of Food and Agriculture 88 (13): 2231–2237.
Wang Y, McAllister TA, Yanke LJ and Cheeke PR, 2000. Effect of steroidal saponins from Yucca schidigera extract on ruminal microbes. Journal Applied Microbial 88: 887-896.
Wencelov M, Varadyova Z, Mihalikova K, Cobanova K, Placha I, Pristas P, Jalac D and Kisidayova S, 2015. Rumen fermentation pattern, lipid metabolism and the microbial community of sheep fed a high-concentrate diet supplemented with a mix of medicinal plants. Small Ruminant Research 125: 64-72.
Woodward A and Reed JD, 1997. Nitrogen metabolism of sheep and goats consuming Acacia brevispica and Sesbania sesban. Journal of Animal Science 75: 1130-1139.
Wright AG and Klieve, AV, 2011. Does the complexity of the rumen microbial ecology preclude-methane mitigation. Animal Feed Science and Technology 166: 248-253.
Yang WZ, Ametaj BN, He ML, Benchaar C and Beauchemin KA. 2010. Cinnamaldehyde in feedlot cattle diets: intake, growth performance, carcass characteristics, and blood metabolites. Journal of Animal Science. 88: 1082-1092.
Yeh YY and Liu L, 2001. Cholestrol-Lowering effect of garlic extracts and organosulfur compounds: human and animal studies. Journal Nutrition 131: 989-993.
Yesilbag D, Biricik H, Cetin I, Kara C, Meral Y, Cengiz SS, Orman A and Udum D, 2016. Effects of juniper essential oil on growth performance, some rumen protozoa, rumen fermentation and antioxidant blood enzyme parameters of growing Saanen kids. Journal Animal Physiology and Animal Nutrition 101(5): 1-10.
Zamani Z, Alipour D, Moghimi HR, Mortazavi SA and Zolhavarieh SM, 2015. Effect of free thymol and sustained release thymol on rumen fermentation and plasma metabolites in sheep. Animal Production Research 3(4): 75-88.
Zhang DF and Yang HJ, 2012. Combination effects of nitrocompounds, pyromellitic diimide, and 2-bromoethanesulfonate on in vitro ruminal methane production and fermentation of a grain-rich feed. Journal Agriculture Food Chemistry 60: 364–371.
Animal Science Research
Volume 32, Issue 3
March 2022
Pages 19-34

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History

  • Receive Date: 13 December 2020
  • Revise Date: 25 September 2021
  • Accept Date: 26 September 2021

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