The effect of hCG injection on serum and seminal plasma testosterone in Ghezel ram

Document Type : Research Paper

Authors

Abstract

BACKGROUND: Reproductive system is affected by hormones so we can control livestock reproduction process by using synthetic hormones and use maximum of their reproductive capacity. OBJECTIVES: The aim of this study was to evaluate the effect of hCG injection on serum and seminal plasma testosterone. METHODS: In this study, six 3-4 years old Ghezel rams were used. Rams was studied with an injection of 500 IU hCG hormone in reproductive season and hCG injection were repeated every seven days for 9 weeks. Blood samples were taken before injections and 30, 60 and 90 minutes after intramuscular injection of hCG from jugular vein. Ejaculate samples were collected at a distance of zero, 1 and 7 days after injection of hCG and serum and seminal plasma testosterone were determined by using ELISA kits. RESULTS: The results showed that hCG injections increased testosterone levels in seminal plasma and serum that serum testosterone levels at 90 minutes after injection of hCG reached to peak, but between 30 and 60 minutes after injection had the greatest increase. Seminal plasma testosterone levels reached the peak value 1 day after injection but 7 days after injection was reduced and close to the base. CONCLUSIONS: As a result, hCG injections can improve reproductive traits by increasing serum testosterone.

References

دقیق کیا حسین، مقدم غلامعلی و وفایی غلامرضا. 1385. فیزیولوژی تولید مثل در حیوانات مزرعه ای.انتشارات دانشگاه تبریز. جلد اول.
Attal J, 1970. Mesure des oestrogènes et des androgens testiculaires et plasmatiques dans l'espèce ovine par des microméthodes de Chromatographie en phase gazeuse: influence de l'âge, de la saison et du cycle diurne. Thèse de Sciences, Paris, No CNRS pp.143.
Bearden j and Fuquay JW, 2003. Applied Animal Reproduction. 6th Edition, Upper Saddle River Pp. 176-195.
Dimov D and Georgiev G, 1977. Ram semen prostaglandin concentration and its effect on fertility. J Anim Sci 44:1050-4.
Hales DB, 2003. Immune Endocrine interactions in the control of Leydig cell function: an overview of research in the Hales' laboratory. J Endocrinol 10: 201-217.
Illius AW, Haynes NB, Purvis K and Lamming GE, 1976. Plasma concentrations of testosterone in the developing ram in different social environments. J Reprod Fert 48: 17-24.
Kishk WH, 2008. Interrelationship between ram plasma testosterone level and some semen characteristics. Slivak J Anim Sci 41: 67-71.
Oliveira Souza LM, Andrade AFC, Celeghini ECC, Negrão JA and Arruda RP, 2011. Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay. R Bras Zootec 12: 2721-2724.
Pavlovich CP, King P, Goldstein M and Schlegel PN, 2001. Evidence of a treatable endocrinopathy in infertile men. J Urol 165:837-41.

Purvis K, Illius AW and Haynes NB, 1974. Plasma Testostrone concentrations in the ram. J Endocrinol 61:241-253.

  Sanford LM, Palmer WM and Howland BE, 1977. Changes in the profiles of serum LH, FSH and Testosteone, and in mating performance and ejaculate volume in the ram during the ovine breeding season. J Anim Sci 45: 1382-1391.
Senger PL, 2005. Pathways to pregnancy and parturition, 2nd rev ed.
Shore L, Yehuda R, Marcus SH, Bartoov B and Shemesh M, 2003. Effect of hCG injection on prostaglandin E concentration in ram seminal plasma. PGs and lip med 70: 291-301.
Sundby A and Torjesen PA, 1978. Plasma levels of testosterone in bulls, Response to repeated hCG injection. Acta Endocrinol 88: 784-92.
Taha TA, Abdel-Gewad EI and Ayoub MA, 2000. Monthly variation in some reproductive parameters of Braki and Awassi rams throughout 1 year under subtropical conditions1. Semen chararacteristics and hormonal levels. J Anim Sci 71: 317-24.
Walkden-Brown SW, Martin GB and Restall BJ, 1999. Role of male–female interaction in regulating reproduction in sheep and goats. J Reprod Fertil 52: 243-257.
Wang C, 1999. Male reproductive function. Kluwer Academic Publisher. Pp.6-11.
Zamiri MJ and Khodaei HR, 2005. Seasonal thyroidal activity and reproductive characteristics of Iranian fat-tailed rams. Anim Reprod Sci 88: 245-255.
Animal Science Research
Volume 26, Issue 2
September 2016
Pages 131-139

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  • Receive Date: 10 September 2016
  • Accept Date: 10 September 2016

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