Investigation the effect of steam and alkaline hydrogen peroxide treatment on the nutritional value of sugarcane top for finishing lambs

Document Type : Research Paper

Authors

1 Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran

2 Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, P.O. Box 63517-73637, Mollasani, Ahvaz, Iran

Abstract

Introduction: A significant portion of livestock feed resources are roughage and fibrous in Iran, which is low in protein, minerals, and digestible energy; however, if treated by some methods, has the potential to be used as an energy source in livestock diets (Adesogan et al. 2019). There are 10 sugarcane development companies in Khuzestan province, which have 127,000 hectares under sugarcane cultivation and the area under sugarcane cultivation is 100,000 hectares per year (FAOSTAT 2013). Sugarcane is harvested in two ways: black (burning) and green (harvesting with a harvester). About 15 to 25 percent of the weight of sugarcane includes leaves and branches named sugarcane top, so the green harvesting produces about 20000 kg of wet biomass per hectare (Moradi et al. 2019). Due to the area under sugarcane cultivation in the Khuzestan province, and its 50% humidity, about two million tons of sugarcane top is produced each year. During harvesting, the sugarcane residues remain on the ground and cause special problems for the maintenance operation of the sugarcane on the field, so the residue is burned in two stages before and after harvesting, and it pollutes the environment, especially the air (Kingston et al. 2005). If the sugarcane top residues treated, due to breakdown of lignocellulose structure and degradation of lignin, cased to the increased access of ruminal microorganisms to lignosullose materials, so instead of burning, they can be used as fodder sources in animal feeding (Karp et al. 2013). Therefore, the aim of present experiment was conducted to evaluate the effects of steam and alkaline hydrogen peroxide treatment to improve the nutritional value of sugarcane top for fattening lambs.
Material and method: The present experiment was performed in Agricultural Sciences and Natural Resources University of Khuzestan. The sugarcane top used in the present experiment was from variety of CP73 and CP57 harvested from Imam Khomeini agro-industry company of Shushtar. After the sugarcane was harvested, it was dried and chopped into 2 to 3 cm long with a chopper and transferred to the Shoaibiyeh Livestock Feed Company for steam treatment. Steam was conducted at 184ᵒC for 4 minutes; the equal pressure was 16 to 18 atm. (average 17 atm.). The sugarcane top was treated using a solution of 1% hydrogen peroxide, which pH was adjusted to 11.5 using a solution of sodium hydroxide (alkaline hydrogen peroxide). Twenty-four Arabi male lambs with average live weight of 30.4 ± 5 kg were used in a completely randomized design with 3 treatments and 8 replicates for a period of 70 days. Experimental treatments were: 1- control (untreated Sugarcane top), 2- diet containing steam treated Sugarcane top, and 3- diet containing steam and alkaline hydrogen peroxide treated sugarcane top.
Results and discussion: Treatment with steam and alkaline hydrogen peroxide significantly reduced the percentage of NDF, ADF, crude fiber, lignin, and increased the significance of the gross energy of sugarcane (P <0.05), but had no effect on crude protein. In consistent with the results of the present experiment, other researchers also reported a decrease in dry matter, NDF, and ADF by applying water vapor under pressure. The decrease of these nutrients has been attributed to the fragmentation or hydrolysis of all or part of the hemicellulose. In compared to control, average feed intake, digestibility of nutrients, final weight, average daily gain, feed efficiency of lambs, rumen propionate and total volatile fatty acids, and blood glucose were increased with feeding of treated sugarcane top, and treatment 3 shown the best results (P<0.05). The components of the experimental diets are the same, and the only difference was in the type of treatments. Therefore, perhaps the reason for the increase in feed consumption in treatments containing sugarcane top treated with steam or steam and alkaline hydrogen peroxide is to improve its digestibility. Increasing digestibility will cause the material to pass through the rumen faster and thus animals consume more feed. The concentration of alanine transaminase was decreased, because of treatment of sugarcane top (P<0.05). The concentration of rumen ammonia nitrogen and pH, blood aspartate transaminase, and carcass traits were not affected by the treatments. Therefore, treatment by steam and alkaline hydrogen peroxide caused to improve the nutritional value of sugarcane top, and their combined use (third treatment) showed better results.

Results and discussion: Treatment with steam and alkaline hydrogen peroxide significantly reduced the percentage of NDF, ADF, crude fiber, lignin, and increased the significance of the gross energy of sugarcane (P <0.05), but had no effect on crude protein. In consistent with the results of the present experiment, other researchers also reported a decrease in dry matter, NDF, and ADF by applying water vapor under pressure. The decrease of these nutrients has been attributed to the fragmentation or hydrolysis of all or part of the hemicellulose. In compared to control, average feed intake, digestibility of nutrients, final weight, average daily gain, feed efficiency of lambs, rumen propionate and total volatile fatty acids, and blood glucose were increased with feeding of treated sugarcane top, and treatment 3 shown the best results (P<0.05). The components of the experimental diets are the same, and the only difference was in the type of treatments. Therefore, perhaps the reason for the increase in feed consumption in treatments containing sugarcane top treated with steam or steam and alkaline hydrogen peroxide is to improve its digestibility. Increasing digestibility will cause the material to pass through the rumen faster and thus animals consume more feed. The concentration of alanine transaminase was decreased, because of treatment of sugarcane top (P<0.05). The concentration of rumen ammonia nitrogen and pH, blood aspartate transaminase, and carcass traits were not affected by the treatments. Therefore, treatment by steam and alkaline hydrogen peroxide caused to improve the nutritional value of sugarcane top, and their combined use (third treatment) showed better results.

Keywords


AOAC. Official methods of analysis, 2005. 18th ed. Association of Official Analytical Chemists, Washington DC, USA.
Adesogan AT, Arriola KG, Jiang Y, Oyebade A, Paula EM, Pech-Cervantes AA, Romero JJ, Ferraretto LF and Vyas D, 2019. Symposium review: Technologies for improving fiber utilization. Journal of Dairy Science 102: 5726–5755.
Aruwayo A, Maigandi SA, Malami BS and Daneji AI, 2011. Haematological and biochemical parameters of Uda lambs fed graded levels of alkali -treated Neem kernel cake. Nigerian Journal of Basic and Applied Science 19: 277-284.
Broderick GA and Kang JH, 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science 63: 64-75.
Cameron MG, Fahey Jr GC, Clark JH, Merchen NR and Berger LL. 1990. Effects of feeding alkaline hydrogen pemxide-treated wheat straw-based diets on digestion and production by dairy cows. Journal of Dairy Science 73:3544.
Chaji M, Mohammadabadi T and Aghaei A. 2011. Fermenting cell walls of processed sugarcane pith by ruminal bacteria, protozoa and fungi. International Journal of Agriculture & Biology 13: 283–286.
Chaji M, Naserian A,Valizadeh R and Mohammadabadi T. 2013. Physicochemical properties of steam treated sugarcane pith and some roughages and their role in ruminants nutrition. Iranian Journal of Animal Science Research 4(4): 298-309.
Chaji M, Naserian AA, Valizadeh R, Mohammadabadi T and Mirzadeh Kh. 2010. Potential use of high-temperature and low temperature steam treatment, sodium hydroxide and an enzyme mixture for improving the nutritional value of sugarcane pith. South African Journal of Animal Sciences 40: 22–31.

Das H, Ali MA, Devi LI, Kirthika P, Gali JMR and Behera P. 2017. Studies on some important enzymes of non-descript sheep of Assam. Journal of Livestock Science 8: 59-62

FAOSTAT. 2013. World agricultural data, http://apps.fao.org/faostat/.
Fisher AV and Boer Hde. 1994. The EAAP standard method of sheep carcass assessment. Carcass measurements and dissection procedures report of the EAAP working group on carcass evaluation, in cooperation with the CIHEAM Instituto Agronomico Mediterraneo of Zaragoza and the CEC Directorate General for Agriculture in Brussels. Livestock Production Science 38: 149-159.
França DA, Longo KM, Turibio Gomes SN, Santo JC, Saulo R, Freitas Bernardo FT, Rudorff EVCEA and Andrade Carvalho Jr. 2012. Pre-harvest sugarcane burning: determination of emission factors through laboratory measurements atmosphere 3: 164-180.
Karp SG, Adenise Lorenci Woiciechowski AL, Soccol VT and Soccol CR. 2013. Pretreatment strategies for delignification of sugarcane bagasse: A Review. Brazilian Archives of Biology and Technology 56: 679-689.
Khanifer H. 2019. The effect of alkaline hydrogen peroxide treatment on nutritional value of palm kernel powder in fattening lambs. MSc Dissertation Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani-Ahvaz.
Kingston G, Donzelli JL, Meyer JH, Richard EP, Seeruttun S, Torres J and Van R. 2005. Impact of the green-cane harvest production system on the agronomy of sugarcane. Proceedings International Society of Sugar Cane Technologists 15: 521-533.
Lewis SM, Kerley MS, Fahey GC, Berger Jr LL and Gould JM. 1987. Use of alkaline hydrogen peroxide treated wheat straw as an energy source for the growing ruminant. Nutrition Reports International 35:1093.
Liu JX, Orskov ER and Chen XB. 1999. Optimization of steam as method for upgrading rice straw as feeds. Animal Feed Science and Technology 76: 345-35.

Meeske R, Meissner HH and Pienaar JP. 1993. The upgrading of wheat straw by alkaline hydrogen peroxide treatment: The effect of NaOH and H2O2 on the site and extent of digestion in sheep. Animal Feed Science and Technology 40. (2–3): 121-133.

Menke KH and Steingass H, 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28: 6-55.
Mohammadabadi T, Chaji M and Bojarpour M. 2012. The effect of processing of sugarcane pith with steam on gas production parameters by using isolated rumen microbiota. Iranian Journal of Animal Science Research 4: 240-246. (Farsi).

Moradi R, Siadat SAA, Siahpoosh AR, Bakhshandeh AM and Moraditalavat MR. 2019. Comparison of quantity and quality indices in green and burnt harvesting sugarcane cultivars. Journal of Crop Production 12: 99-110.

Muzamal M, Baath JA, Olsson L and Rasmuson A. 2016. Contribution of structural modification to enhanced enzymatic hydrolysis and 3-D structural analysis of steam-exploded wood using X-Ray tomography. BioResources 11: 8509-8521.
Ottenstein D and Bartley D. 1971. Improved gas chromatography separation of free acids C2-C5 in dilute solution. Analytical Chemistry 43: 952-955.
Sharifi A and Chaji M. 2019. Effects of processed recycled poultry bedding with tannins extracted from pomegranate peel on the nutrient digestibility and growth performance of lambs. South African Journal of Animal Science 49: 290-300.
UlAin HB, Saeed F, Arshad MU, Ahmad N, Nasir MA, Amir RM, Kausar R and Niaz B. 2018. Modification of barley dietary fiber through chemical treatments in combination with thermal treatment to improve its bioactive properties. International Journal of Food Properties 21: 2491–2499.
Van Soest PJ, Robertson JB and Lewis BA. 1991. Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583–3597.
Xing Y, Su Z, Wang K, Deng L and Jiang J. 2014. Combination of low-pressure steam explosion and alkaline peroxide pretreatment for separation of hemicellulose, BioResources 9: 3384-3395.
Zahedifar M, Karimi N, Fazaeli H and Mirhadi SA. 2014. Optimization of steam treatment conditions for improving the nutritive value of date leaves. Journal of Agricultural Science and Technology 16: 811-825.