Effect of olive pomace on digestibility, blood biochemical parameters and milk production and composition of dairy Buffalo

Document Type : Research Paper

Authors

1 Ramin Agriculture and Natural Resources University of Khuzestan

2 Agricultural Sciences and Natural Resources University of Khuzestan,

3 Agricultural Sciences and Natural Resources University of Khuzestan

Abstract

Background and objective: Since, feeding in animal husbandry is one of the most critical and costly issues, therefore should focus on cheap resources like agricultural wastes which provide adequate nutrients for livestock. Olive pomace is a residue of the oil extraction of olive fruit, which after drying can be used as a by-product in animal feed. Olive pomace contains high lignin (32% DM), cellulose and hemicellulose (Sadeghi et al., 2009). Raw olive pomace contains high amounts of 16 and 18 fatty acids; and about 96% of olive pomace fatty acids include linoleic acid and oleic acid (Nazari et al., 2008). Olive pomace is containing anti-nutritional components such as phenolic compounds and tannins (0.58 to 1.1 g/kg DM), and it may have adverse effects on livestock (Molina et al, 2003). Feeding dairy ruminants with non-core olive cake containing tocopherol, retinol and phenols can cause positive changes on milk quality, and improved oxidative stability of milk (Pauselli et al, 2007). Most of buffalo breeders use wheat flour in buffalo diet. Using wheat flour, can destroy the rumen microbial population and as an important part in human foods, increases costs. However, there is no research on wheat flour replaced with olive pomace in buffalo nutrition. Therefore, this study was conducted to explore the effect of olive cake on digestibility, blood biochemical parameters and production performance of Khuzestani dairy Buffalo.
Material and methods: Fifteen dairy buffaloes average 4 years, average body weight 570 kg and 2 parities were used in a completely randomized design with 3 treatments and 5 repetitions. Experimental treatments were included 0%, 7.5%, and 15% olive pomace substituted with wheat flour, which fed two times daily for 45 days in a completely randomized design. Diets were formulated according to the NRC. The olive pomace used in this study dried by air drying. The pomaces were stirred and mixed several times daily. The crude protein, metabolizable energy, neutral detergent fiber, acid detergent fibers, and fat of olive pomace were 6.5%, 2.65 Mk / kg DM, 56, 45, and 9 %, respectively.
At the end of the experiment, feed intake, nutrient digestibility, production performance and blood parameters were measured. The diets were prepared completely mixed and given daily at 7:00 and 17:00. Fresh water was also available to them. The combination of control diet consisted of corn silage, wheat straw, olive cake, wheat flour, wheat bran, rice bran, mineral supplement and vitamins, limestone. Milking was done every day in the morning and evening and milk production was recorded. Milk scanner was used to measure milk protein, fat, and lactose. The milk pH determined by using pH papers or electrical pH meters; and milk solids and non-fat solids (SNF), by lactometer.
Also, blood samples were taken from all buffaloes at the end of the period, 3 to 4 hours after morning intake and blood metabolites determined. For apparent digestibility of the diets, during three consecutive days, buffalo feces were collected. Also, sampling of feed residuals in the last three days of the period was weighed. Then, the digestibility of organic matter, dry matter, crude protein, crude fat, acid detergent fibers and neutral detergent fibers were measured.
The obtained data were analyzed using the General Linear Model procedure of the SAS. A comparison of the mean was made with Duncan. The model of this design was a completely randomized design with three treatments, and each treatment includes five replications based on the statistical model: Yij= µ + Ti+ eij. Also, repeated data per time were analyzed based on the mixed statistical model. Yci = µ + Tc + Ɛic + (T*P)ik.
Results: Milk fat and SNF increased in experimental treatments at the end of the period (P<0.05). However, milk production, protein, acidity, density and pH of milk were not affected by the experimental treatments (P>0.05). Milk production increased by using dried olive kernels in dairy buffaloes (Terramoccia et al., 2013). Inclusion of unsaturated fatty acids often reduces the saturated fatty acids of milk. Also, high fat in olive pomace can be a major reason for high percentage of milk fat in the olive pomace treatment compared to control (Chilofalo et al. 2003). Also, the concentration of blood parameters was not affected by experimental diets. But, blood cholesterol in 7.5% olive cake increased and the concentration of alkaline phosphatase enzyme increased by 15% olive cake compared to the other treatments (P<0.05). Obeidat (2017) reported that adding olive pomace to the diet of Awasi lambs had no effect on blood parameters (glucose, blood urea nitrogen, aspartate aminotransferase and alanine aminotransferase). But Hashish and Al-Sameh (2012) declared that saturated fatty acids in olive pomace can increase the concentration of saturated fatty acids and blood cholesterol. Other researchers also concluded that with the increase in dietary fat concentration, blood cholesterol increased (Bhatta et al., 2011). Nutrient digestibility was not affected by experimental treatments (P>0.05). Using olive pomace up to 20% instead of barley in Lori lambs increased microbial protein production in the rumen. Also, olive pomace in the Dalagh sheep diet increased feeding efficiency and carcass weight; and reduced production costs up to 66% (Samadi, 2009). The use of olive pomace in Awasi lambs, did not influence dry matter intake, digestibility of crude protein, neutral detergent fiber and acidic detergent fiber (Obeidat, 2017).
Conclusion: According to the recent study, replacing wheat flour with olive cake had no negative effect on production, blood parameters and digestibility of nutrients in dairy buffaloes, and due to the reasonable price of olive cake; replacing olive cake up to 15% with wheat flour in the diet of dairy buffaloes is recommended.

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Abdeddou S, Rischkowsky B, Richter EK, Hess HD and Kreuzer M, 2011. Modification of milk fatty acid composition by feeding forages and agro-industrial by-products from dry areas to Awassi sheep. Journal of Dairy Science, 94:4657–4668.
Aberghani A, 2007. The effect of replacing beet pulp instead of barley on the yield and characteristics of carcasses of Moghani lambs. Master's thesis. Ramin Agricultural and Natural Resources University.
Agricultural Statistics, 2005. Ministry of Agriculture Jihad, Office of Statistics and Information Technology, Volume 1, Crops and Horticulture, 2004, 1st edition, Tehran.
Amini N, Kazemi M, Chenari M and Khaltabadi Farahani A, 2015. The effect of different sources of omega-3, omega-6 and L-carnitine on reproductive function and some blood parameters during the flushing period of Farahani ewes. Animal Production Research. 14,7.
AOAC,1990. Official Method of Analysis.15. Association of official analytical chemists. Arlington, VA, USA.
AOAC, 2000. Official Method of Analysis. 16. Association of official analytical chemists Washington, DC. USA.
Bauman DE And Griinari JM, 2003. Nutritional Regulation of Milk Fat Synthesis. Annual Review of Nutrition 23: 203-227.
Baumgard LH, Corl BA, Dwywr DA, Saeb A and Bauman DE, 2000. Identification of the conjugated linoleic acid hatinhibits milk fat synthesis. American Journal Physiology. Isomer Reglatory IntegrativeComparative Physiology 278: 179-184.
Baumgard LH, Sangster JK and Bauman DE, 2001. Milk fat synthesis in dairy cows is progressively reduced by increasing supplemental amounts of trans-10, cis-12 conjugated linoleic acid (CLA). Journal of Nutrition 131: 1764-1769.
Bell AW and Bauman DE, 1997. Adaptations of glucose metabolism during pregnancy and lactation. Journal of Mammary Gland Biology and Neoplasia 2: 265-278.
Bernard F, 2013. Effect of crude olive cake supplementation on camel milk production and fatty acid composition. Dairy Science and Technology, 93: 225-239.
Bhatta R, Vaithiyanathan S, Singh NP, Shinde AK and Verma DL, 2000. Effect of feeding tree leaves as supplements on the nutrient digestion and rumen fermentation pattern in sheep grazing semi-arid range of India. I. Small Ruminant Research 60: 273–280.
Chilliard Y, Glasser F, Ferlay A, Bernard L, Rouel J and Doreau M, 2007. Diet, rumen biohydrogenation and nutritional quality of cow and goat milk fat. Eur J Lipid Sci Technol 109: 828–855.
Chilofalo B, Liotta L, chiofalo V and Zumbo A, 2002. Lasansa d’oliva nell’alimentazione degli ovini: effetto sulla composizione acidica del latte (olive cake for ewe feeding: effect on the milk acidic composition). P136-137. In: proceedings of the 15th national Congress of S.I.P.A.O.C. Cagliari, Italy.
Delbecchi L, Ahnadi C, Kennelly J and Lacasse P, 2001. Milk fatty acid composition and mammary lipid metabolism in Holstein cows fed protected or unprotected canola seeds. J. Dairy Sci 84: 1375-1381.
Doosti F, Ghoorchi T, Sepahvand A, Dasdar B and Azarfar A, 2016. The effect of different levels of olive cake on fermentation parameters, rumen microbial protein in male lambs. Iranian Journal of Animal Science Research 4(9): 424-436.
Eghbali H, Mohammadabadi T, Chaji M, Bujarpour M and Islami M, 2014. The effect of ascophylum nodousum brown algae on milk composition, digestibility, fermentation and respiratory rate of Holstein lactating cows in khuzestan. Iranian Veterinary Journal, 11,2.
Esmaili H, Hojabri F, Moeini MD and Hajjarian E, 2016. Effects of replacing roasted soybeans with roasted rapeseed seeds in the diets of lactating cows on the production yield and composition of milk fatty acids. Journal of Research in Ruminants 4:4.167-188.
Fahey GC and Berger LL, 1988. Carbohydrate nutrition of ruminants. In: Church, D.C. (Ed.) The Ruminant Animal Digestive Physiology and Nutrition. Prentice-Hall International UK. pp. 269-297.
Geravand A, Chaji M, Sari M and Mohammadabadi T, 2015. The effect of olive cake on digestibility, fermentation characteristics and rumen protozoa population of Arab sheep. Journal of Animal Science Research. 4:26.
Ha LY, Grimm NK and Pariza MW, 1989. Newly recognized anticarcinogenic fatty acids: identification and quantification in natural and processed cheeses. J. Agric. Food Chem 37: 75–81.
He M and Armentano LE, 2011. Effect of fatty acid profile in vegetable oils and antioxidant supplementation on dairy cattle performance and milk fat depression. J Dairy Sci 94: 2481–2491.
Hally well B, 1987. Oxidants and human diseases. Some new concepts. Fas Journal 1: 441-445.
Hashish SM and El-Samee LD, 2012. Olive cake and barley malt in hen diets to improve egg lipids and fatty acids. Iranian Journal of Applied Animal Science 2(4): 383-389.
Hervas G, PilarFrutos F, Javier Giraldez Angel R, ManteconMaria C and AlvarezDel P, 2003. Effect of different doses of quebracho tannins extract on rumen fermentation in ewes. Animal feed Science and Technology 109: 65-78.
Karim A, Diani A and Khalaji A, 2006. Milk and its quality. University of Tehran. Second edition.
Mandrekar P and Szabo G, 2009. Signalling pathways in alcohol-induced liver inflammation. Journal of Hepatology 50(6): 1258-1266.
Min BR, Attwood GT, McNabbb WC, Molanb AL and Barry TN, 2005. The effetct of condensed tannins from Lotus corniculatus on the proteolytic activities and growth of rumen bacteria. Animal Feed Science and Technology, 121: 45-58.
Min BR, Pinchak WE, Fulford JD and Puchala R, 2005. Effect of feed additives on in vitro and in vivo rumen characteristics and frothy bloat dynamics in steers grazing wheat pasture. Animal Feed Science and Technology 123:  615–629.
Mohammed R, McGinn SM and Beauchemin KA, 2011. Prediction of enteric methane output from milk fatty acid concentrations and rumen fermentation parameters in dairy cows fed sunflower, flax, or canola seeds. Journal of Dairy Science, 94: 6057–6068.
Mohamadi R, Farokhi F, Najati V and AsriRezaei S, 1392. Assessment of the Amount of Hepatohistopatological and Enzymatic Changes after Chronic Lead Intoxication in Utero and Throughout Life in Rat. Qom Univercity Medical Science Journal, 7(1): 83-90.
Molina AE, Yanez RD, Moumen A and Martin GI, 2003.Composition and nitrogen availability for goats and sheep of some olive by-products. Small Ruminant Research, 49: 329-336.
Nazari B, Asgari P, Sarrafzadegan N, Saberi S, Haji Hosseini R and Ezhari Ah, 2009. Evaluation of fatty acid composition in olive oil and animal oil with emphasis on trans fatty acids by gas chromatography. Journal of Shahrekord University of Medical Sciences, 40(4):57-63.
NRC, 2001. Nutrient Requirements of Dairy Cattle. 7th rev. ed. Natl. Acad. Press, Washington, DC.
Obeidat B, 2017. The effects of feeding olive cake and Saccharomyces cerevisiae supplementation on performance, nutrient digestibility and blood metabolites of Awassi lambs. Animal Feed Science and Technology  231: 131-137.
Palmquist DL, Lock AL, Shingfield KJ and Bauman DE, 2005. Biosynthesis of conjugated linoleic acid in ruminants and humans. Pages 179–217 in Advances in Food and Nutrition Research. Vol. 50. S.-L. Taylor, ed. Elsevier Academic Press, San Diego, CA.
Pauselli M, Servili M, Esposto S, Gervasi G, Mourvaki E, Taticchi A, Urbani S, Selvaggini R, Concezzi L and Montedoro G, 2007. Effect of destoned olive cake as animal feed on ewe milk quality. Proc. Int. Conf. “New technologies for the treatment and valorization of agro by-products”, ISRIM, Terni, 3-5 Oct. 2007.
Perez E, Vera R, Aguilar C, Lira R, Pena I, Valenzuela A and Cerda H, 2013. Effect of dietary inclusion of lampante olive oil on milk and cheese fatty acid profiles of ewes. grasas y aceites, 64 (3), 295-303.
Rouhani M, Mortazavi A and Jouyandeh H, 2015. Milk technology and dairy products. Ferdowsi University of Mashhad.
Sabri Gol, Mahmut K and Serafettin K, 2010. Olive cake usage as an alternative to Cotton seed meal in dairy goat feeding. African Journal of Agricultural Research Vol. 5(13): 1643-1646.
Sadeghi H, Tempura Ynsari A and Ansari-pirsarai Z, 2009. Effects of different olive cake by products on dry matter intake, nutrient digestibility and performance of zel sheep. International Journal of Agriculture and Biology 11: 39-43.
Samadi F and Shams Shargh M, 2008. Chemical composition and digestibility of olive cake using live animals. Journal of Agricultural Sciences and Natural Resources, 15: 3.
Shahi M and Ghourchi T, 2016. The effect of different levels of whole cottonseed on production, milk compounds, digestibility and blood parameters in dairy cows. Iranian Journal of Animal Science Research 4,8: 625-635.
Shakker RR, Jumah RY and Abu- jdayil B, 2000. Reological properties of plain yoghurt during coagulation process: Impact of fat content and preheat treat ment of milk. Journal of food Engineering 44: 175- 180.
Shingfield KJ, Bonnet M and Scollan ND, 2013. Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7(Suppl. 1):132–162.
Szumacher-Strabel M, Cieslak A and Nowakowaska A, 2009. Effect of oils rich in linoleic acid on in vitro rumen fermentation parameters of sheep, goats and dairy cows. Journal of Animal and Feed Sciences 18: 440-452.
Taghizadeh A, Mahbooub S, Pourabbas S, Moghadam G, Safamehr A and Paya H, 2009. The effect of cottonseed in the diet of Holstein dairy cows on milk production and composition and pattern of milk fatty acids and conjugated linoleic acid production. Journal of Animal Science Research.19 (2). 85-95.
Tamime AY and Robinson RK, 1999. Yoghurt, Science and Technology. Cambridge, UK: woodhead publishing limited.
Teimouri Yansari A, Sadeghi H, Ansari-Pirsarai Z and Mohammad-Zadeh H, 2007. Ruminal dry matter and nutrient degradability of different olive cake by-products after incubation in the rumen using Nylon bag technique. International Journal of Agriculture and Biology 9: 439-442.
Terramoccia S, Bartocci S, Taticchi A, Di Giovanni S, Pauselli M, Mourvaki E, Urbani S and Servili M, 2013. Use of dried stoned olive pomace in the feeding of lactating buffaloes: Effect on the quantity and quality of the milk produced. Asian-australas. Journal of Animal Science, 26:971–980.
Van Soest PJ, 1994. Function of the ruminant forestomach. Pages 230–252 in Nutritional Ecology of the Ruminant. 2nd ed. Cornell University Press, Ithaca, NY.
World Health Organization (WHO), 2003. Diet, nutrition and the prevention of chronic diseases. Report of a Joint WHO/FAO Expert Consultation. WHO Technical Report Series No. 916. WHO, Geneva, Switzerland.