استفاده از دانه کلزا به عنوان منبع چربی در جیره‌های بر پایه ذرت و جو بر پاسخ‌های عملکردی و متابولیکی بز نژاد مهابادی در اوایل شیردهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی دانشگاه ارومیه

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه

چکیده

زمینه مطالعاتی: بهبود عمکرد و بررسی متابولیت‌های شکمبه‌ای و خونی بزهای نژاد مهابادی. هدف: بررسی اثر استفاده از دانه کلزا به عنوان منبع چربی در جیره‌های بر پایه ذرت و جو (به عنوان منابع مختلف نشاسته) بر پاسخ های عملکردی و متابولیکی بز نژاد مهابادی در اوایل شیردهی. روش‌ کار: این آزمایش بر روی 16 راس بز ماده نژاد مهابادی در مرحله‌ی اوایل شیردهی به صورت فاکتوریل 2×2 در قالب طرح آماری کاملاً تصادفی با دو فاکتور منبع غلات و دانه کلزا، صورت گرفت. تیمارهای آزمایشی شامل جیر‌ه‌ بر پایه‌ی جو، جیره بر پایه‌ی ذرت، جیره‌ی بر پایه‌ی جو به همراه 5 درصد دانه کلزا و جیره‌ی بر پایه‌ی ذرت به همراه 5 درصد دانه کلزا بود. میانگین تیمارها به روش آزمون توکی در سطح 5 درصد مقایسه شدند. نتایج: افزودن دانه کلزا بر ماده خشک مصرفی معنی‌دار نبود. قابلیت هضم ماده خشک، ماده‌ی آلی، پروتئین خام و چربی خام معنی‌دار نبود ولی مقدار الیاف نامحلول در شوینده‌ی خنثی و اسیدی کاهش یافت(05/0>P). اثر افزودن دانه کلزا بر غلظت کلسترول و کل پروتئین خون معنی‌دار بود و باعث افزایش این فرآسنجه‌ها شد (05/0>P)؛ اما در غلظت آلبومین، اوره، تری گلیسیرید و گلوکزخون تغییر معنی‌داری مشاهده نشد. ترکیب شیر تحت تاثیر تیمارها معنی دار نبود ولی تولید شیر در تیمار ذرت به همراه کلزا و جو به همراه کلزا افزایش معنی‌داری نشان داد (05/0>P). سطح pH مایع شکمبه تغییر نکرد؛ اما جمعیت پروتزآی شکمبه‌ای کاهش معنی‌داری نشان داد (05/0>P). هرچند از میان اسیدهای چرب فرار مایع شکمبه، مقدار ایزووالریک و والریک افزایش یافت (05/0>P)، اما در مقدار اسید پروپیونیک و اسیدبوتیریک تغییر معنی‌داری مشاهده نشد. نتیجه‌گیری نهایی: نتایج نشان داد که افزودن سطح 5 درصد دانه کلزا به عنوان منبع چربی در جیره می‌تواند سبب بهبود عملکرد بزهای مهابادی در اوایل دوره‌ی شیردهی شود.

کلیدواژه‌ها


عنوان مقاله [English]

The use of cannula seed as a source of fatty acid in the diet based on corn and barley on functional responses and some metabolite in early lactation Mahabadian goats

نویسندگان [English]

  • Nouradeen Aryan 1
  • Younes Ali Alijoo 2
  • Behzad Asadnezhad 1
1 Urmia University
2 Department of animal science, Agriculture faculty, Urmia University
چکیده [English]

Abstract
Background and objective: Milk and other dairy products are important sources of human nutrition for providing protein, vitamins and minerals. Milk fat contains substances known to have anti-cancer properties. Fat milk ruminants due to extensive bio hydrogenation of unsaturated fatty acids in the rumen and the synthesis of Short and medium saturated fatty acids in the chest glands have a high concentration of saturated fatty acids. Goat is grown as one of the domestic ruminants in different parts of the world. This livestock produces a variety of products. One of the main goals of breeders is milk production. The small, high adaptability, low tolerance and high resistance of goats to many diseases and unfavorable conditions in the environment have caused the maintenance and cultivation of this animal, especially in rural and small conditions. One of the strategies for livestock breeding is livestock nutrition, which greatly affects the economic aspects of production. The lactating animals during and after the transition period undergo significant changes in the metabolism of glucose, fatty acids and minerals. In order to meet the metabolic needs of livestock, different nutritional strategies have been used in this period. Therefore, feeding non forage carbohydrates and fat in the diet or reducing the percentage of milk fat at the start of lactation through some specific fatty acids to reduce energy demand in early lactation it has been used.
This experiment was conducted to investigate the effect of using Cannula seed as a source of fatty acid in corn and barley diets on the functional and metabolic responses of the Mahabadian goat breeder in early lactation. Therefore, the use of fat in the diet has become a management tool in controlling metabolic events around childbirth.
Materials and methods: The treatments consisted of diet based on barley, corn-based diet, barley-based diet with 5 per centage Cannula seed and corn with 5 per centage Cannula seed diet. Treatments were given to TMR as livestock. This experiment was carried out on 16 female Mahabadian goat breeders in the early stage of lactation in a 2 × 2 factorial arrangement in a completely randomized design ( CRD) with two source factors of cereals and Cannula seed. The mean of treatments was compared with Duncan test at 5 per centage level. This study was conducted in a one-month trial period after birth and 14 days of adolescence. During the experimental period, the amount of feed intake and weight gain was recorded. Sampling of feeds to determine dry matter intake and nutrient feedings weekly to measure the amount of organic matter, dry matter, crude protein, insoluble fiber in neutral detergent ( NDF) and Insoluble fiber in acidic detergent ( ADF). In order to determine the blood parameters of the blood sample from the veins and veins of the goats on the final day of the test, 4 hours after the morning meal was taken. The blood samples taken were immediately transferred to the laboratory and after centrifugation at 6000 rpm for 7 minutes, the serum was isolated and kept in the freezer at -20 ° C. Separated serum was used to measure the triglycerides, cholesterol, urea nitrogen, total protein and glucose, using Pars-test and ELISA reader made by Garni Company in Germany. In order to determine the rumen parameters, a sample of rumen fluid in the final day of the test, 4 hours after feeding. The pH of the rumen fluid was measured immediately using pH meter (Schott Titrator Titroline easy model). Rumen fluid samples were smoothed using 4 layer sackcloth and 2 samples of 50 ml of rumen fluid with 1 ml sulfuric acid 50 per centage with a ratio of 1 to 50 sulfuric acid with rumen fluid was mixed to determine the amount of ammonia nitrogen in the rumen fluid and the profile of volatile fatty and immediately and kept in the freezer at -20 ° C until further tests were performed.
Results: addition of canola to feed was not significant on dry mater intake. The apparent digestibility of DM, OM, CP and EE was not significant, but the amount of NDF, ADF decreased. The effect of fatty acid on total cholesterol and total protein was significant and increase this metabolite but no significant changes were observed in albumin, urea, triglyceride and glucose concentrations. Milk composition was not significantly affected by treatments but milk production in corn with canola, barley with canola increased significantly. The pH of the rumen fluid did not change, but the amount of protozoa decreased. The amount of iso-voleric and voleric acid increased from ruminal fluid escaping VFA, but there was no significant change in the amount of propionic acid and butyric acid.

Conclusions: According to the results, it can be concluded that the use of Cannula seed as a source of fatty acid in a grain-based diet improves the performance of lactating goats. Considering that in the majority of studies done in the feeding of oily seeds, a significant decrease in feed intake was reported. Therefore, it seems that using 5 per centage of Cannula seed in corn and barley not only causes Reducing dry matter consumption does not even increase milk production. Therefore, the use of 5 per centage is recommended in grain-based rations at the lactation stage.
The results of this study showed addition of Cannula seed as a source of fatty acid in the diet improved the performance of the Mahabadian goats in the early stages of lactation.

کلیدواژه‌ها [English]

  • Lactation
  • Mahabadian Goat
  • cannula seed - Blood Metabolites
Abu-Ghazaleh AA, Potu RB and Ibrahim S, 2009. Short communication: The effect of substituting fish oil in dairy cow diets with docosahexaenoic acid-micro algae on milk composition and fatty acids profile. Journal of Dairy Science 92: 6156-6159.
AbuGhazaleh AA, Schingoethe DJ, Hippen AR, Kalscheur KF and Whiltlock LA, 2002. Fatty acid profiles of milk and rumen digesta from cows fed fish oil, extruded soybeans or their blend. Journal of Dairy Science 85:2266-2276.
Ackers MR, 2002. Lactation and the Mammary Gland. Iowa State Press, Ames, Iowa: 95.
AOAC, 2002. Official Methods of Analysis. 52th Ed. AOAC International, Gaithersburg, Maryland, USA.
Aranda-Avila I, Herrera-Camacho J, Ake-Lopez J R, Delgado-Leon R A, Ku-Vera J C, 2010. Effect of supplementation with corn oil on postpartum ovarian activity, pregnancy rate, and serum concentration of progesterone and lipid metabolites in F1 (Bos-taurus × Bos-indicus) cows. Tropical Animal Health and Production 42: 1435–1440.
Bauman DE and Griinari J M, 2003. Nutritional regulation of milk fat synthesis. Annual Review of nutrition 23:203–227.
Bhatt R, Soren N, Tripathi M and Karim S, 2011. Effects of different levels of coconut oil supplementation on performance, digestibility, and rumen fermentation and carcass traits of Malpura lambs. Journal of Animal Feed Science and Technology 164: 29–37.
Blanco C, Giráldez F J, Prieto N, Morán L, Andrés S, Benavides J, Tejido ML and Bodas R, 2014. Effects of dietary inclusion of sunflower soap stocks on nutrient digestibility, growth performance, and ruminal and blood metabolites of light fattening lambs. Journal of Animal Science 92: 4086-4094.
Cheng  KJ,  Forsberg  CW,  Minato  HJ, Costerton  W,  1991.  In:  Tsuda  T, Sasaki Y, Kawashima  R  (Eds.), Physiological Aspects of Digestion and Metabolism  in  Ruminants,  Academic  Press, New York pp. 596–624.
Chichlowski MW, Schroeder JW, Park CS, Keller WL and Schimek DE, 2005. Altering the fatty acids in milk fat by including canola seed in dairy cattle diets. Journal of Dairy Science 88: 3084-3094.
Delbechi L, Ahnadi CE, Kennelly JJ and Lacasse P, 2001. Milk fatty acid composition and mammary lipid metabolism in Holstein cows fed protected or unprotected canola seed. Journal of Dairy Science 84:1375-1381.
Demirel G, Wachira AM, Sinclair LA, Wilkinson RG, Wood JD and Enser M, 2004. Effects of dietary n-3 polyunsaturated fatty acids, breed and dietary vitamin E on the fatty acids of lamb muscle, liver and adipose tissue. British Journal of Nutrition 91: 551–565.
DePeters E, German J, Taylor S, Essex S and Perez-Monti H, 2001. Fatty acid and triglyceride composition of milk fat from lactating Holstein cows in response to supplemental canola oil. Journal of Dairy Science 84: 929-936.
Doepel L, Lapierre H and Kennelly JJ, 2002. Peripartum performance and metabolism of dairy cows in response to prepartum energy and protein intake. Journal of Dairy Science  85:2315–2334.
Doreau M and Chilliard Y, 1997. Effect of ruminal or post ruminal fish oil supplementation on intake and digestion dairy cows. Repord Nutrition 37: 113-124.
FASS ,2010. Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching. 3rd rev. ed. Federation of Animal Sciences Societies Savoy, IL.
Funston RN, 2004. Fat supplementation and reproduction in beef females. Journal of Animal Science 82:154-161
Glasser F, Ferlay A and Chiliard Y, 2008. Oil seed lipid supplements and fatty acid composition for cow milk: A Meta-ANALYSIS. Journal of Dairy Science 91:4687-4703.
González-Paramás A M, Esteban-Ruano S, Santos-Buelga C, de Pascual-Teresa S and Rivas-Gonzalo J C, 2004. Flavanol content and antioxidant activity in winery by products. Journal of Agricultural Food Chemestry  52:234–238.
Gozho GN, Hobin MR and Mutsvangwa T, 2008. Interactions between barley grain processing and source of supplemental dietary fat on nitrogen metabolism and urea-nitrogen recycling in dairy cows. Journal of Dairy Science 91:247–259.
Harfoot CG and Hhazlewood GP, 1997. Lipid metabolism in the rumen. Pages 382-426 in the rumen microbial Ecosistem, Hobson PN and Stewart CS, ed. Chapman and Hall, London UK.
Harvatine KJ and Allen MS, 2006. Effects of fatty acid supplements on milk yield and energy balance of lactating dairy cows. Journal of Dairy Science 89 1081-1091.
Hess BW, Moss GE and Rule DC, 2008. A Desade of developments in the area of fat supplementation research with beef cattle and sheep. Journal of Animal Science 86: 188-204.
Jenkins TC, 1993. Lipid metabolism in the rumen. Journal of Dairy Science 76:3851-3863.
Luginbuhl JM, Poore MH and Conrad AP, 2000. Effect of level of whole cottonseed on intake, digestibility and performance of growing male goats fed hay-based diets. Journal of Animal Science 78:1677-1683.
Manso T, Castro T, Mantecón AR and Jimeno V, 2006. Effects of palm oil and calcium soaps of palm oil fatty acids in fattening diets on digestibility, performance and chemical body composition of lambs. Journal of Animal Feed Science and Technology 127: 175–186.
 McAllister TA, Cheng KJ, Rode LM and Forsberg CW, 1990. Digestion of barley, maize, and wheat by selected species of ruminal bacteria.  Applied and Environmental Microbiology 56:3146.
National Research Council (NRC), 2001. Nutrient Requirement of Dairy Cattle, 7th revised ed. National Academy of Science, Washington DC.
Onetti SG, Shaver RD, McGuire MA and Grummer RR, 2001. Effect of type and level of dietary fat of rumen fermentation and performance of dairy cows fed corn silage-based diets. Journal of Dairy Science. 84:2751-2759.
Ottenstein DM and Batler DA, 1971. Improved gas chromatography separation of free. Acids C–C in dilute solution. Anal Chemestry 43: 952-955.
Overton TR and Waldron MR, 2004.  Nutritional Management of Transition Dairy Cows: Strategies to Optimize Metabolic Health. Journal of Dairy Science 87:105–E119.
Petit H, Germiquet V and Lebel D, 2004. Effect of feeding whole, unprocessed sunflower seeds and flaxseed on milk production, milk composition, and prostaglandin secretion in dairy cows. Journal of Dairy Science 87:3889-3898.
Robinson RS, Pushpakumara PG, Cheng Z, Peters AR, Abayasekara DR and Wathes DC, 2002. Effects of dietary polyunsaturated fatty acids on ovarian and uterine function in lactating dairy cows. Reproduction 124:119-131.
SAS Guide, SAS. 2002. Version 9.1. SAS Institute Inc, Cary NC.
Shingfield KJ, Bonnet M and Scollan ND, 2010. Recent developments in altering the fatty acid composition of ruminant derived foods. Animal 7: 132–162.
Van Keulen J and Young BA, 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science 44: 282–287.
Whitlock LA, Schingothe DJ, Abughazaleh AA, Hippen AR and Kalscheur KF, 2006. Milk production and compositin from cows fed small amounts of fish oil and soybeans. Journal of Dairy Science 89:3972-3980.
Zened A, Enjalbert F, Nicot MC and Troegeler-Meynadier A, 2013. Starch plus sunflower oil addition to the diet of dry dairy cows results in a trans-11 to trans-10 shift of biohydrogenation. Journal of Dairy Science 96:451-459.
Zhang XD, Chen WJ and Liu JX, 2009. Effects of protein-free energy supplementation on blood metabolites, insulin and hepatic PEPCK gene expression in growing lambs offered rice straw-based diet. Czech Journal of Animal Science 54: 481-489.