Detection of carrier cattle infected to Theileria annulata by molecular assay in northwest of Iran

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه پاتوبیولوژی-دانشکده دامپزشکی- دانشگاه تبریز

2 گروه پاتوبیولوژی، دانشکده دامپزشکی ، دانشگاه تبریز

چکیده

Bovine theileriosis due to Theileria annulata is one of the most important diseases in livestock animals in the world. The disease is transmitted by Ixodidae ticks and characterized by fever, enlargement of lymphatic glands, cachexia and death. The detection of theileriosis is enforcement by the blood smear staining with Giemsa. These techniques are suited diagnosing acute infection but are not in carrier animals, where infection rate may be little. The aim of present study was to the detection of carrier cattle to T. annulata in blood samples by PCR and comparison with the staining method in the northwest of Iran. Materials and Methods: A total number of 281 peripheral blood samples were obtained from apparent healthy cattle of different ages and in native and crossbreed cattle in the northwest of Iran in summer 2019. Samples were obtained from animals showing no clinical signs. The age range of cattle was 1-8 years. Samples were detected by Giemsa staining and light microscopic observation and PCR method based on applying the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams-1) gene. A chi-square test was performed to compare prevalence related to breed and age categories. Results: In the microscopic method 25 samples (8.89%), were positive. However, the PCR detected 108 samples (38.43 %) positive for T. annulata. In positive samples of cattle, the highest prevalence was recorded in cattle 2-5 years old (22.4%). These differences in age results were significant (p <0.05). Out of 108 samples positive by PCR, 45 samples (41.66%) were native cattle and, 63(58.33%) were crossbreed cattle, whereas this difference was not significant. Conclusion: Our results showed that there is a high percentage of carriers cows in northwest of Iran and indicate a high potential risk for the infestation of healthy animals and vectors of the disease.
زمینه مطالعاتی : تیلریوز گاوی ناشی از تیلریا آنولاتا یکی از بیماریهای مهم در حیوانات مزرعه در جهان می باشد. این بیماری توسط کنه های ایکسودیده منتقل شده و با تب، بزرگی غدد لنفاوی ، لاغری مفرط و مرگ مشخص می شود. تشخیص این بیماری بر اساس رنگ آمیزی گسترشهای خونی به روش گیمسا انجام می گیرد. این روش برای تشخیص عفونت حاد مناسب می باشد اما برای تشخیص حاملین بیماری که میزان آلودگی آنها کمتر می باشد مناسب نیست. هدف: این مطالعه جهت تشخیص حاملین بیماری ناشی از تیلریا آنولاتا توسط نمونه های خونی و مقایسه آن با روش مولکولی PCR در گاوان شمالغرب کشور انجام پذیرفت. روش کار : تعداد 281 نمونه خونی از گاوان بومی و دو رگه به ظاهر سالم با سنین مختلف در شمالغرب کشور در تابستان سال 1398 اخذ شد. نمونه ها بعد از تهیه گسترش و رنگ آمیزی به روش گیمسا در زیر میکروسکپ نوری بررسی شدند و روش واکنش زنجیره ای پلیمراز (PCR) با استفاده از پرایمر های تهیه شده از آنتی ژن سطحی مروزوئیت انگل که ژن (Tams-1) را کد می کنند انجام شد. آزمون مربع کای جهت جهت مقایسه میزان شیوع بیماری در ارتباط با نژاد و سن حیوانات کار گرفته شد. نتایج: در روش میکروسکپیک تعداد 25 نمونه (89/8%) نمونه ها از لحاظ آلودگی انگلی مثبت تشخیص داده شد ولی در روش PCR 108 نمونه (43/38%) نمونه ها مثبت تشخیص داده شد. در نمونه های مثبت گاوان بیشترین میزان شیوع در گاوان 5-2 ساله (4/22 %) مشاهده شد که این تفاوت معنی دار بود(0،05>P) . در بین 108 نمونه مثبت تشخیص داده شده با PCR 45 مورد (66/41 %) در گاوان بومی و 63 مورد (33/58 %) در گاوان دورگه شناخته شد. نتیجه گیری: نتایج بررسی حاضر میزان بالای گاوان حامل بیماری تیلریوزیس را نشان داد که این موضوع نشانگر پتانسیل بالای خطر آلودگی برای حیوانات سالم و ناقلین بیماری می باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Detection of carrier cattle infected to Theileria annulata by molecular assay in northwest of Iran

نویسندگان [English]

  • Ahmad Nematollahi 1
  • Parisa Shahbazi 2
  • Samad Ebrahimian 2
  • Belal Hassanzadeh 2
1 عضو هیات علمی
2 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz
چکیده [English]

Bovine theileriosis due to Theileria annulata is one of the most important diseases in livestock animals in the world. The disease is transmitted by Ixodidae ticks and characterized by fever, enlargement of lymphatic glands, cachexia and death. The detection of theileriosis is enforcement by the blood smear staining with Giemsa. These techniques are suited diagnosing acute infection but are not in carrier animals, where infection rate may be little. The aim of present study was to the detection of carrier cattle to T. annulata in blood samples by PCR and comparison with the staining method in the northwest of Iran. Materials and Methods: A total number of 281 peripheral blood samples were obtained from apparent healthy cattle of different ages and in native and crossbreed cattle in the northwest of Iran in summer 2019. Samples were obtained from animals showing no clinical signs. The age range of cattle was 1-8 years. Samples were detected by Giemsa staining and light microscopic observation and PCR method based on applying the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams-1) gene. A chi-square test was performed to compare prevalence related to breed and age categories. Results: In the microscopic method 25 samples (8.89%), were positive. However, the PCR detected 108 samples (38.43 %) positive for T. annulata. In positive samples of cattle, the highest prevalence was recorded in cattle 2-5 years old (22.4%). These differences in age results were significant (p < 0.05). Out of 108 samples positive by PCR, 45 samples (41.66%) were native cattle and, 63(58.33%) were crossbreed cattle, whereas this difference was not significant. Conclusion: Our results showed that there is a high percentage of carriers cows in northwest of Iran and indicate a high potential risk for the infestation of healthy animals and vectors of the disease.
زمینه مطالعاتی : تیلریوز گاوی ناشی از تیلریا آنولاتا یکی از بیماریهای مهم در حیوانات مزرعه در جهان می باشد. این بیماری توسط کنه های ایکسودیده منتقل شده و با تب، بزرگی غدد لنفاوی ، لاغری مفرط و مرگ مشخص می شود. تشخیص این بیماری بر اساس رنگ آمیزی گسترشهای خونی به روش گیمسا انجام می گیرد. این روش برای تشخیص عفونت حاد مناسب می باشد اما برای تشخیص حاملین بیماری که میزان آلودگی آنها کمتر می باشد مناسب نیست. هدف: این مطالعه جهت تشخیص حاملین بیماری ناشی از تیلریا آنولاتا توسط نمونه های خونی و مقایسه آن با روش مولکولی PCR در گاوان شمالغرب کشور انجام پذیرفت. روش کار : تعداد 281 نمونه خونی از گاوان بومی و دو رگه به ظاهر سالم با سنین مختلف در شمالغرب کشور در تابستان سال 1398 اخذ شد. نمونه ها بعد از تهیه گسترش و رنگ آمیزی به روش گیمسا در زیر میکروسکپ نوری بررسی شدند و روش واکنش زنجیره ای پلیمراز (PCR) با استفاده از پرایمر های تهیه شده از آنتی ژن سطحی مروزوئیت انگل که ژن (Tams-1) را کد می کنند انجام شد. آزمون مربع کای جهت جهت مقایسه میزان شیوع بیماری در ارتباط با نژاد و سن حیوانات کار گرفته شد. نتایج: در روش میکروسکپیک تعداد 25 نمونه (89/8%) نمونه ها از لحاظ آلودگی انگلی مثبت تشخیص داده شد ولی در روش PCR 108 نمونه (43/38%) نمونه ها مثبت تشخیص داده شد. در نمونه های مثبت گاوان بیشترین میزان شیوع در گاوان 5-2 ساله (4/22 %) مشاهده شد که این تفاوت معنی دار بود(0،05>P) . در بین 108 نمونه مثبت تشخیص داده شده با PCR 45 مورد (66/41 %) در گاوان بومی و 63 مورد (33/58 %) در گاوان دورگه شناخته شد. نتیجه گیری: نتایج بررسی حاضر میزان بالای گاوان حامل بیماری تیلریوزیس را نشان داد که این موضوع نشانگر پتانسیل
بالای خطر آلودگی برای حیوانات سالم و ناقلین بیماری می باشد.
ovine theileriosis due to Theileria annulata is one of the most important diseases in livestock animals in the world. The diseases is transmitted by Ixodidae ticks and characterized by fever, enlargement of lymphatic glands, cachexia and death. The detection of theileriosis is enforcement by the blood smear staining with Giemsa. These techniques are suited diagnosing acute infection but are not in carrier animals, where infection rate may be little. The aim of present study was to the detection of carrier cattle to T. annulata in blood samples by PCR and comparison with the staining method in the northwest of Iran. Materials and Methods: A total number of 281 blood samples were obtained from healthy cattle of different ages and in native and crossbreed cattle in the northwest of Iran in summer 2019. Sampling was achieved from animals showing no clinical signs. The age range of cattle was 1-8 years. Samples were detected by Giemsa staining and light microscopic observation and PCR method based on applying the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams-1) gene. A chi-square test was performed to compare prevalence related to breed and age categories. Results: In the microscopic method 25 samples (8.89%), were positive. However, the PCR detected 108 samples (38.43 %) positive for T. annulata. In positive samples of cattle, the highest prevalence was recorded in cattle 2-5 years old (22.4%). These differences in age results were significant (p < 0.05). Out of 108 samples positive by PCR, 45 samples (41.66%) were native cattle and, 63(58.33%) were crossbreed cattle, whereas this difference was not significant. Conclusion: Our results showed a high percentage of carriers in cattle northwest of Iran and indicate a high potential risk for the infestation of healthy animals and vectors of the disease.

کلیدواژه‌ها [English]

  • Theileria annulata
  • carrier
  • PCR
  • staining method
  • Iran

مراجع

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پژوهش های علوم دامی (دانش کشاورزی)
دوره 30، شماره 4
اسفند 1399
صفحه 95-101

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  • تاریخ دریافت: 28 آبان 1399
  • تاریخ بازنگری: 16 دی 1399
  • تاریخ پذیرش: 26 اسفند 1399

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