بررسی اثر اشکال مختلف سلنیوم بر خصوصیات هضمی خوراک کاملا مخلوط گاوهای پر‌شیر، با استفاده از روش‌های آزمایشگاهی و کیسه‌های نایلونی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی دانشکده کشاورزی دانشگاه تبریز

2 گروه علوم دامی دانشگاه تبریز

چکیده

زمینه مطالعاتی: سلنیوم یکی از عناصر معدنی کم مصرف می‌باشد که برای عملکرد بهتر حیوانات ضروری است. هدف: تحقیق حاظر به منظور ارزیابی اثر اشکال مختلف سلنیوم بر خصوصیات هضمی خوراک کاملا مخلوط‌ گاوهای پر‌شیر، با استفاده از روش های آزمایشگاهی انجام گرفت. روش کار: آزمایش در 3 دوره 28 روزه شامل 21 روز عادت‌دهی و 7 روز نمونه‌برداری اجرا شد. تیمارها شامل: 1) جیره پایه+ ppm 3/0 سلنیوم بصورت نانو، 2) جیره پایه+ppm 3/0سلنیوم بصورت سلنومتیونین، 3) جیره پایه+ ppm 3/0 سلنیوم بصورت سلنیت‌سدیم بودند. در روش تولید‌گاز 300 میلی‌گرم از هر نمونه در ساعات 2، 4، 6، 8، 12، 16، 24، 36، 48، 72 و 96 انکوباسیون گردید. در روش کیسه های نایلونی ( کیسه هایی با ابعادcm 6×12) مقدار 5 گرم نمونه به مدت صفر، 2، 4، 8، 12، 16، 24، 36، 48 ،72 ساعت در شکمبه سه رأس گوسفند انکوبه شد. در روش سه مرحله ای هضم حدود 5 گرم از خوراک در کیسه‌هایی به ابعادcm 10× 5 قرار داده و 12 ساعت در شکمبه گوسفندان انکوبه گردید. نتایج: در طول اکثریت قریب به اتفاق ساعات انکوباسیون بیشترین میزان تولید گاز به ترتیب در تیمار نانو، آلی، معدنی و کنترل مشاهده شد. این اختلافها از ساعات 36 انکوباسیون به بعد معنی دار بودند. پتانسیل تولید‌گاز (A) در تیمارهای نانو، آلی و معدنی بیشتر از شاهد بودند (05/0p <). بین تیمارنانو با آلی اختلاف معنی داری از لحاظ پتانسیل تولید گاز وجود نداشت. تفاوت معنی‌داری بین تیمارها، از نظر تجزیه‌پذیری ماده خشک خوراک کاملا مخلوط وجود نداشت. در آزمایش سه مرحله‌ای هضم، تیمار نانو موجب افزایش تجزیه‌پذیری بعد شکمبه‌ای ماده خشک خوراک کاملا مخلوط نسبت به تیمار معدنی شد (05/0p <). نتیجه گیری نهایی: نتایج نشان می‌دهد که افزودن منابع مختلف سلنیوم به جیره‌ می‌تواند عملکرد شکمبه را بهبود بخشد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of different forms of selenium on digestibility of high production lactating dairy cows totally mixed ration using in vitro and in situ techniques

نویسندگان [English]

  • S Dehghani 1
  • A Taghizadeh 2
  • A Hoseinkhani 1
  • H Mohammadzadeh 1
چکیده [English]

Introduction: Selenium is one these trace mineral elements which is essential for health, immunity and maximum production performance of animals. Selenium is an essential trace element, and its importance for animal health and productivity has been well confirmed. Selenium has known to be involved in enzyme activity and preventing oxidative damage to body tissue. Selenium plays important roles in antioxidant defence systems, prevents cell damage and is necessary for growth, fertility, and immune system in farm animals. Recently, nano -elemental Se has attracted wide spread attention due to its high bioavailability and low toxicity, because nanometer particulates exhibit novel characteristics, such as great specific surface area, high surface activity, a lot of surface active centres, high catalytic efficiency and strong adsorbing ability and over and above the character of low toxicity of Se0. Dietary selenium is an essential trace element for animals and humans with a variety of biological functions. It plays important roles in the regulation of thyroid hormone metabolism, cell growth and antioxidant defence systems thus, together with alpha-tocopherol prevents cells against oxidative stress damage, also these compounds are necessary for growth, fertility, and immune system health in animals and humans. The in situ and in vitro usually use for determining of effect of additives on digestibilities of t feeds. Regarding to the importance of TMR feeding on dairy cattle nutrition, determining of its digestibilities with additive and without additive is necessary. The aim of this study was to investigate the effects of different forms of selenium sources on digestibility of high production lactating dairy cows TMR in vitro and in situ Techniques.
Materials and Methods: 3 male ruminally cannulated sheep, average 43±4.8 kg of BW, were used in a replicated 3×3 Latin square experiment. Sheep were fed twice daily (08:00 and 18:00 h) at maintenance nutrition requirements with a basal diet consisting of 400 g/kg (dry matter) DM of basal concentrates and 600 g/kg DM of forage. Sheep were placed in metabolic cages individually and fresh water was freely available during the experimental period. This experiment was conducted in three periods of 28 days with 21 d adaptation period and 7 d for data tacking. Treatments were: 1. Basal diet + 0.3 ppm nano selenium, 2. Basal diet + 0.3 ppm organic selenium, 3. Basal diet + 0.3 ppm inorganic selenium. In gas production method, 300 mg of each treatment weighted and incubated for 2, 4, 6, 8, 12, 16, 24, 36, 48, 72, 96 hours. The Nylon bag method used to estimate ruminal disappearance of dry matter. Ruminal degradability was measured using nylon bag technique on day 22–28 of the experimental period. The samples 5 g of feed were weighed in 6 cm×12cm nylon bags made of monofilament Pecap polyester . Samples were incubated separately in duplicate bags and suspended in the rumen of each sheep and removed after 0, 2, 4, 8, 12, 16, 24, 36, 48 and 72 hours. All removed bags were rinsed in cold water until the bags were clean; the bags immediately were dried in an oven at 65◦C for 12 h, then 105 ◦C for 24 h in order to determine DM disappearance. in three steps digestion method, about 5 g of feed stuffs weighted in 6 cm×10cm nylon bags made of monofilament Pecap polyester . Samples were incubated separately in duplicate bags and suspended in the rumen of each sheep and removed after 12 hours. All removed bags were rinsed in cold water until the bags were clean; the bags immediately were dried in an oven to determine DM disappearance.
Results and Discussion: During most of incubation hours, the volume of produced gas was higher in nano selenium, organic selenium, inorganic selenium and control treatments, respectively. However, significant differences between treatments were occurred after 36 h of incubation. The most gas production potential (A) was measured in nano selenium, organic selenium and inorganic selenium compared with control treatments. There were not any significant differences between nano selenium and organic selenium in gas production potential. there wasn’t any significate differences between treatments in degradability of TMR dry matter. In three steps digestion experiment, nano treatment was higher than inorganic treatment in post ruminal digestion of TMR dry matter.
CONCLUSIONS: The results showed that the addition of various sources of selenium to the diet may improve rumen function.

کلیدواژه‌ها [English]

  • Selenium
  • Totally mixed ration
  • Gas production
  • Nylon bag
  • Three steps digestion
  • Dairy cow
AOAC, 2002. Official Methods of Analysis. 17th Edn. AOAC International, Gaithersburg, Maryland, USA.
Barceloux DG, 1999. Selenium. Journal of Toxicology - Clinical Toxicology 37:145-172.
Blummel M and Ørskov ER, 1993. Comparison of gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Animal Feed Science and Technology 40: 109–119.
Chaudhary M, Garg AK, Mittal GK and Mudgal V, 2009. Effect of organic selenium supplementation on growth, se uptake, and nutrient utilization in guinea pigs.  Biological Trace Element Research 133:217–226.
Del Razo-Rodriguez OE, Ramirez-Bribiesca JE, Lopez-Arellano R, Oblitas F, Contreras PA, Bohmwald H and Wittwer F, 2000. Efecto de la suplementacion con selenio sobre la actividad sanguínea de glutation peroxidasa (GSH-PX) y ganancia de peso en bovinos selenio deficientes mantenidos a pastoreo. Archivos De Medicina Veterinaria 32:55-62.
Fedorak PM and Hrudey SE, 1983. A Simple apparatus for measuring gas production by methanogenic cultuvesin serum bottles. Environmental Technology Letters 4: 425-435.
Gargallo S, Calsamiglia S and Ferret A, 2006. Technical note: A modified three-step in vitro procedure to determine intestinal digertion of proteins. Journal of Animal Science 84:2163-2176.
Getachew G, Makkar HPS and Becker K, 2002. Tropical browses: content of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acids and in vitro gas production. Journal of Agricultural Science 139: 341-352.
Hidiroglou M, Heaney DP and Jenkins KJ, 1968. Metabolism of inorganic selenium in rumen bacteria. Canadian Journal of Physiological Pharmacology 46, 229–232
Kim J, Vansoest PJ and Combs GF, 1997. Studies on the effects of selenium on rumen microbial fermentation in vitro. Biological Trace Element Research 56:203-213.
Kojouri GA and Shirazi A, 2007. Serum concentration of Cu, Zn, Fe, Mo, and Co in newborn lambs following systemic administration of Vitamin E and selenium to the pregnant ewes. Small Ruminant Research 70:136-139.
McDougall EI, 1948. The composition and output of sheep in saliva. Biological Chemical Journal 43: 99-109.
Mohri M, Ehsani A, Norouzian MA, Heidarpour M and Seifi HA, 2011. Parenteral selenium and vitamin E supplementation to lambs: hematology, serum biochemistry, performance, and relationship with other trace elements. Biological Trace Element Research 139:308-316.
Najafnejad B, Aliarabi H, Taghizadeh A and Alipour D, 2014. Comparison effects of different selenium sources in cottonseed rich diets on digestibility of the diet, performance and hematological parameters of lactating dairy cows. Journal of Ruminant Research 2:79-98.
Najafnejad B,  Aliarabi H, Tabatabaei MM, Taghizadeh A, Alipour D and Zaboli KH, 2016. Effects of different sources of selenium on some hematological parameters and antioxidant response in Holstein dairy cows. Journal of Animal Science Research 26: 45-57.
NRC, 1985. Nutrient Requirements of Sheep, 5th edn. National Academy of Sciences, Washington, DC.
NRC, 2001. Nutrient Requirements of Dairy Cows. 7th edn. National Academy of Sciences, Washington, DC.
NRC, 2007. Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids. National Academy Press,Washington, DC.
Opatpatanakit Y, Kellaway RC, Lean IJ, Annison G and Kirby A, 1994. Microbial fermentation of cereal grains in vitro. Australian Journal of Agricultural Research 45: 1247-1263.
Ramirez BJE, Tortora JL, Huerta M, Aguirre A and Hernandez LM, 2001. Diagnosis of selenium status in grazing dairy goats on the Mexican plateau. Small Ruminant Research 41:8-85.
Schrauzer GN, 2000. Selenomethionine: a review of its nutritional significance, metabolism and toxicity. The Journal of Nutrition 130: 1653–1656.
Schrauzer GN, 2003. The nutritional significance, me­tabolism and toxicology of selenomethionine. Advances in Food and Nutrition Research 47: 73–112.
Shi L,  Xun W, Yue W, Zhang C, Ren Y, Liu Q, Wang Q and Shi L, 2010. Effect of elemental nano-selenium on feed digestibility, rumen fermentation, and purine derivatives in sheep. Animal Feed Science and Technology 163:136–142.
Shi LG, Xun WJ, Yue WB, Zhang CX, Ren YS, Liu Q, Wang Q and Shi L, 2011. Effect of elemental nano-selenium on feed digestibility, rumen fermentation, and purine derivatives in sheep. Animal Feed Science and Technology 163:136–142.
Underwood EJ and Suttle NF, 1999. The mineral nutrition of livestock. CAB international, Wallingford, U.K.
Wang C, Liu Q, Yang WZ, Dong Q, Yang XM, He DC, Zhang P, Dong KH and Huang YX, 2009. Effects of selenium yeast on rumen fermentation, lactation performance and feed digestibilities in lactating dairy cows. Livestock Science 126: 239–244.
Xu BH, Xu ZR and Xia MS, 2003. Effect of nano red elemental selenium on GPx activity of broiler chick kidney cells in vitro. Wuhan University Journal of Natural Sciences 8: 1167–1172.
Xun W, Shi L, Yue W, Zhang CH, Ren Y, Liu Q, 2012. Effect of High-Dose Nano-selenium and Selenium–Yeast on Feed Digestibility, Rumen Fermentation, and Purine Derivatives in Sheep. Biological Trace Element Research 150:130–136.
Zhang JS, Wang XF, Xu TW, 2008. Elemental selenium at nano size (Nano-Se) as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with Se-methylselenocysteine in mice. Toxicological Sciences 101:22–31.