اثر منابع مختلف سلنیوم بر فراسنجه های خونی و پاسخ آنتی اکسیدانی گاوهای شیری هلشتاین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 1 دانشجوی دکتری تغذیه دام گروه علوم دامی دانشکده کشاورزی دانشگاه بوعلی سینا همدان

2 2 دانشیار گروه علوم دامی دانشگاه بوعلی سینا همدان

3 3 استاد گروه علوم دامی دانشگاه تبریز

4 4 استادیار گروه علوم دامی دانشگاه بوعلی سینا همدان

چکیده

زمینه مطالعاتی: سلنیوم جایگاه ویژه­ای در بین آنتی­اکسیدان­های طبیعی دارد و از اکسیداسیون ساختار­های سلولی جلوگیری می­کند. هدف:این آزمایش به منظور بررسی اثر منابع مختلف سلنیوم بر فراسنجه­های خون و پاسخ آنتی اکسیدانی در گاو­های شیری هلشتاین تغذیه شده با جیره­های غنی از چربی انجام شد. روشکار: 12 راس گاو هلشتاین با میانگین تولید 4±40 کیلوگرم شیر در روز در قالب طرح مربع لاتین ادغام شده ناقص با 4 تیمار و3 تکرار به ازای هر تیمار، مورد استفاده قرار گرفتند. آزمایش در 3 دوره 28 روزه با 4 تیمار شامل: 1) جیره پایه محتوی 20% پنبه دانه بدون مکمل سلنیوم؛ 2) جیره پایه + 3/0 قسمت در میلیون سلنیوم معدنی، سلنیت سدیم؛ 3) جیره پایه  +3/0 قسمت در میلیون سلنیوم آلی، مخمر غنی از سلنیوم و 4) جیره پایه  +3/0 قسمت در میلیون نانو سلنیوم) اجرا شد. میزان تولید شیر ثبت شد و در روز­های صفر و پایانی­ هر دوره، نمونه­گیری از خون انجام شد. نتایج: نتایج نشان داد که افزودن سلنیوم اگرچه تغییری در مصرف ماده خشک ایجاد نکرد اما باعث افزایش تولید شیر و شیر تصحیح شده برای 4 درصد چربی  شد (05/0P<)، افزودن سلنیوم به جیره تأثیری بر غلظت گلوکز، ازت اوره­ای، کراتنین، پروتئین تام، آلبومین و گلوبولین خون گاو­های شیری نداشت. استفاده از سلنیوم بویژه نوع آلی و نانو آن موجب کاهش غلظت متابولیت­های چربی و فعالیت آنزیم­­های آلکالین فسفاتاز و آسپارتات آمینوترانسفراز سرم و افزایش ظرفیت آنتی­اکسیدانی خون گاو­های شیری شد (05/0P<). نتیجه گیرینهایی :بطور کلی نتایج این مطالعه نشان داد که افزودن 3/0 قسمت در میلیون سلنیوم به جیره­های حاوی سطوح بالایی از منابع چربی غیر اشباع باعث کاهش معنی­دار غلظت چربی­های مضر سرم و افزایش فعالیت آنتی­اکسیدانی آن می­شود، که بازتاب آن در بهبود توان تولیدی گاو­ها مشاهده میشود.
 

کلیدواژه‌ها


عنوان مقاله [English]

Effects of different sources of selenium on some hematological parameters and antioxidant response in Holstein dairy cows

نویسندگان [English]

  • B Najaf nejad 1
  • H Ali arabi 2
  • MM Tabatabaee 2
  • A Tagi zadeh 3
  • D Alipoor 2
  • KH Zaboli 4
چکیده [English]

BACKGROUND: Selenium has a special place in the natural antioxidants and prevents the oxidation of cell structures. OBJECTIVES:This experiment was conducted in order to investigate the effects of different sources of selenium on blood parameters and antioxidant response in Holstein dairy cows fed fat rich diets. METHODS: Twelve Holstein cows with average milk production of 40±4kg were used in merged incomplete Latin squares design with 4 treatments and 3 repeats in each treatment. The experiment was designed and conducted in 3 periods of 28 days and 4 treatments including; group 1 (basal diet containing 20% cottonseed meal, without selenium supplement), group 2 (basal diet+ 0.3 ppm inorganic selenium, as sodium selenite), group 3 (basal diet+0.3 ppm organic selenium, as selenised yeast) and group 4 (basal diet+0.3 ppm selenium, as nano selenium). At the first and last days of each period, blood samples were collected and milk production was also recorded. RESULTS:The results showed that however adding different selenium sources to the diets had no effect on dry mater intake; it increased milk and 4% FCM production. Selenium supplementation did not affect blood glucose concentration, urinary nitrogen, ceratenin, total protein, albumin and globulin concentrations of dairy cows. Selenium supplementaition, specialy organic and nano selenium, significantly decreased concentration of serum fat metabolites and activity of Alkaline phosphatase and Aspartate Amino Transferase activity (p<0.05) and increased blood total antioxidant capacity. CONCLUSIONS:Overall results of the present study showed that supplementation of 0.3 ppm selenium to the diet containing high level of unsaturated fat decreased concentration of serum harmful fats and increased antioxidant capacity of serum which is reflected in improved milk production of the cows.

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