The effect of in ovo feeding of L- methionine on carcass traits, small intestine morphology, and blood metabolites of day-old broiler chicks

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز

2 گروه بافت شناسی، دانشکده دامپزشکی، دانشگاه تهران

چکیده

چکیده
زمینه مطالعاتی: متیونین به عنوان اولین اسید آمینه محدود‌کننده در طیور برای ساخت پروتئین و رشد ضروری است. در چندین مطالعه اثر تزریق درون‌تخم‌مرغی دی‌ال-‌متیونین بررسی شده است، اما در مورد تزریق درون‌تخم‌مرغی ال-‌متیونین گزارشی وجود ندارد. هدف: بنابراین هدف از این پژوهش بررسی اثر تغذیه درون‌تخم‌مرغی ال-متیونین بر وزن، صفات لاشه، ریخت‌شناسی روده‌کوچک و فراسنجه‌های خونی در جوجه‌های گوشتی یک روزه بود. روش کار: به منظور دستیابی به هدف این مطالعه، 240 تخم‌مرغ مادر گوشتی (راس 308) بر اساس طرح کاملا تصادفی با 8 تیمار آزمایشی و 30 تخم‌مرغ در هر تیمار استفاده شد. تیمارهای آزمایشی شامل 6 سطح ال-متیونین (19/0، 38/0، 57/0، 76/0، 95/0 و 14/1 درصد ال- متیونین)، شم شاهد (تزریق آب مقطر) و شاهد (گروه بدون تزریق) بودند که در روز 14 دوره جوجه‌کشی به درون مایع‌آمنیوتیک تزریق شدند. بعد از تفریخ، جوجه‌ها وزن کشی شدند، نمونه‌های خون جمع‌آوری شده و جوجه‌ها کشتار شدند تا صفات لاشه و ریخت‌شناسی روده‌کوچک بررسی شوند. سپس سرم نمونه‌های خونی جمع‌آوری شد و برای اندازه گیری غلظت متابولیت‌های خونی استفاده شد. نتایج: نتایج این پژوهش نشان داد تغذیه درون‌تخم‌مرغی ال-متیونین بازده لاشه و وزن ران، سینه، سنگدان و تیموس را افزایش داد (05/0 p <)، اما اثری بر جوجه‌درآوری نداشت (05/0 P>). همچنین، اثرهای کاهشی تیمارهای ال- میتونین بر گلوکز، تری گلیسرید و نیتروژن اوره‌ای خون مشاهده شد (05/0 p <). علاوه‌بر‌این، تزریق درون‌تخم‌مرغی ال-متیونین موجب بهبود طول و وزن روده‌کوچک و فراسنجه‌های ریخت‌شناسی ژژنوم و دوازدهه شد (طول پرز، نسبت طول پرز به عمق کریپت، قطر کریپت و عمق کریپت)، (05/0 p <). نتیجه گیری نهایی: بر اساس نتایج این پژوهش، تغذیه درون‌تخم‌مرغی 19/0 درصد ال-متیونین اثر بهبود دهنده بر جوجه درآوری، صفات لاشه، فراسنجه‌های خونی، اندام‌های سیستم ایمنی و ریخت‌شناسی روده‌کوچک داشت؛ بنابراین 19/0 درصد ال-متیونین سطح قابل توصیه برای تغذیه درون‌تخم‌مرغی است.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of in ovo feeding of L- methionine on carcass traits, small intestine morphology, and blood metabolites of day-old broiler chicks

نویسندگان [English]

  • B Frdoust 1
  • M Ebrahimi 1
  • Gh Moghaddam 1
  • M Olyaee 1
  • M Adib Moradi 2
  • S Alijani 1
چکیده [English]

Introduction: Methionine, as the first limiting amino acid in poultry, is necessary for protein synthesis and growth. Several studies evaluated the impact of in ovo injection of DL- methionine, but there is no report regarding the in ovo injection of L- methionine. Aim: Therefore, the purpose of this study was to investigate the effect of in ovo feeding of L- methionine on weight, carcass traits, small intestine morphology, and blood metabolites of day-old broiler chicks. Materials and method: To achieve the aim of the study, 240 broiler breeder eggs (Ross 308) were used based on a completely randomized design with eight experimental treatments and 30 eggs per each treatment. Experimental treatments included: six levels of L- methionine (0.19, 0.38, 0.57, 0.76, 0.95, and 1.14% L- methionine), a sham control (injection of distilled water), and a control (non- injected group), which were injected into the amniotic fluid at 14 days of incubation. After hatching, chicks were weighed, blood samples were collected, and they were humanly euthanized to evaluate carcass traits and small intestine morphology. After that, serum of blood samples was collected and used for measuring metabolite concentrations. Results: The results of the study indicated that in ovo feeding of L- methionine increased carcass efficiency and thigh, breast, gizzard, and thymus weight (p < 0.05), but did not influence hatchability (P>0.05). In addition, reducing effects of L- methionine treatments on serum glucose, triglyceride, and blood urea nitrogen were observed (p < 0.05). Moreover, in ovo injection of L- methionine improved length and weight of small intestine, and duodenum and jejunum morphology parameters (villous height, villous height/crypt depth ratio, crypt diameter, and crypt depth), (P <0.05). Conclusion: According to the results of this study, in ovo feeding of 0.19% L- methionine had an improving effect on hatchability, carcass traits, blood metabolites, immune system organs, and morphology of small intestine; therefore, 0.19% L- methionine is an advisable level for in ovo feeding.

مراجع

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پژوهش های علوم دامی (دانش کشاورزی)
دوره 29، شماره 1
خرداد 1398
صفحه 175-188

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  • تاریخ دریافت: 09 شهریور 1398
  • تاریخ پذیرش: 09 شهریور 1398

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